Fig. 5

USF2 affected the proliferation, migration and invasion of GC cells. A Relative expression of USF2 in GC tissues and normal tissues from TCGA. B The survival curve of USF2 in GC patients based on K-M plotter database (http://www.kmplot.com, data from TCGA). (p < 0.001). C CCK8 assays were performed to detect the proliferation of AGS and MKN-45 cells transfected with si-USF2 or si-NC. D EdU assays were performed to detect the proliferation of AGS and MKN-45 cells transfected with si-USF2 or si-NC. E Wound-healing assays were used to detect migration ability of AGS and MKN-45 cells transfected with si-USF2 or si-NC. F Transwell migration and invasion assays were performed to determine the migration and invasion ability of AGS and MKN-45 cells transfected with si-USF2 or si-NC. G Western Blot detected the expression of associated proteins. All experiments were repeated three times. *p < 0.05; Data expressed as mean ± SD