Fig. 7

Demethylation at enhancer region (cg08889930) significantly negatively correlated with NUP37 mRNA expression. A Diagram showing the CpG islands in the promoter region of human NUP37 gene. B Heatmap showing overall methylation patterns in the DNA sequence of human NUP37 gene. C Correlation heatmap visualizing the relationship between NUP37 mRNA expression and the methylation level of CpG island at DNA sequence of NUP37 gene. D–F Scatter plot showing that NUP37 mRNA expression significantly negatively correlated with the methylation level of cg08889930 in HCC patients from 3 independent datasets. G NUP37 mRNA expression in Huh7 cells significantly increased after decitabine treatment *** p < 0.001, based on ANOVA. H NUP37 mRNA expression in Huh7 cells significantly reduced after treatment with CPI-455. **** p < 0.0001, based on ANOVA. I–J Representative immunoblot of HCC cells (Huh7) lysates showing that decitabine increased NUP37 protein expression and CPI-455 decreased NUP37 protein expression. The NUP37, GAPDH and MCM2 (Fig. 6I-J) bands of Huh7 cell lysates were obtained from same gel