Fig. 2

MCM2 and NUP37 are upregulated in human HCC. A Box plots showing MCM2 and NUP37 mRNA expression levels between HCC and nontumor samples from TCGA dataset (TCGA-LIHC, n = 421). B Box plots showing MCM2 and NUP37 protein expression levels between HCC and peri-tumor samples from NODE dataset (OPE00321, n = 330). p value is based on Wilcoxon test. C, D Representative immunoblot of 10 pairs of HCC sample lysates showing that MCM2 and NUP37 were overexpressed in HCC. The MCM2, NUP37, and GAPDH bands of each paired sample group were acquired in the same gel. E MCM2 and NUP37 mRNA expression levels were determined by RT-PCR in 10 pairs of HCC samples. * p < 0.05, ** p < 0.01 by paired Student’s t-test. F, G Box plots showing MCM2 and NUP37 mRNA expression levels between HCC and the paired nontumor tissues samples from GSE57957 and E-MTAB-4171 dataset respectively. H–J MCM2 and NUP37 expression were detected by immunoblot and RT-PCR in human HCC cells and normal liver cells. HCC, hepatocellular carcinoma; TCGA, **** p < 0.0001, based on ANOVA. TCGA, The Cancer Genome Atlas; LIHC, liver hepatocellular carcinoma; NODE, National Omics Data Encyclopedia; RT-PCR, real-time PCR