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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Autoantigen microarrays reveal myelin basic protein autoantibodies in morphea

Fig. 4

Double immunofluorescent staining of mouse brain cortex with human sera and anti-MBP antibodies. Representative images of cortex sections stained with MBP-positive morphea patient serum 400 (A–C), MBP-negative morphea patient serum 199 (D–F), normal human serum (NHS; G–I) and no human serum (J–L) together with commercial anti-MBP antibodies. Human serum staining is shown in green (A, D, G, and J), and MBP staining is shown in red (B, E, H, and K). Merged images (C, F, I, and L) show human serum staining, MBP staining, and nuclear staining with DAPI in blue. Images were taken using a 63X objective. Scale bar (20 µm) shown in panel A applies to all images in the figure. Only MBP-positive morphea patient serum 400 shows robust staining in MBP-rich myelinated axons in a similar pattern (C) compared to MBP-negative patient serum 199 (F), normal human serum (I) or no human serum (L)

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