Fig. 1

Antigen array profiling identifies a distinct autoantibody biosignature in morphea sera. Heatmaps displaying significant serum IgG reactivity associated with (A) morphea cases versus healthy controls or (B) morphea cases versus disease (scleroderma) controls versus healthy controls. Autoimmune microarrays were incubated with diluted sera, autoantibody binding was detected with fluorescently-labeled anti-human IgG secondary antibody, and arrays were scanned to quantify binding to each antigen feature. SAM analysis identified antigen features with statistical differences in reactivity (q < 0.01). Red represents high reactivity, green intermediate reactivity, and blue lack of reactivity