Fig. 3

CCR5 conformation frequency visualized in JC53 cells. Quantified CCR5 subpopulation frequency in JC53 HeLa derivatives transfected with CCR5-GFP fused to the CT, showing total versus surface CCR5 events. Cells were fixed and labeled with an NT or ECL2 epitope-specific AlexaFluor 647-conjugated mAb (A). Cells labeled for total CCR5 events were fixed and permeabilized prior to staining (B). Cells were imaged using a Zeiss LSM 800 microscope and ZEN Blue 2.3 software. CCR5-GFP (green); mAb (red); co-localized signals (yellow). Bar size—5 µm