Fig. 1

Comparison of dual staining CCR5 imaging methods. The C-terminus is GFP tagged (green); the extracellular epitopes are stained with domain specific mAbs (red) (A). A U87.CD4.CCR5-GFP cell expressing CCR5 C-terminus-fused GFP, stained with Alexa 647-conjugated anti-ECL1/ECL2 45523 mAb (B) [50] and a confocal image under the same conditions (C). Bar size—10 µm