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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Immune cell profiles of patients with interstitial cystitis/bladder pain syndrome

Fig. 2

A Images of biopsied tissues from patients with Interstitial Cystitis/Bladder Pain Syndrome with Hunner Lesions (IC/BPS-HL) and unaffected controls (UC) stained for immune cell markers. Cross sections of bladder urothelium viewed at ×40 magnification and stained for Hematoxylin and Eosin (H&E, nucleus/cytoplasm), CD3 (pan T cells), CD20 (B cells), CD138 (plasma cells), CD14 (monocytes), and CD15 (neutrophils/eosinophils). Biopsies were performed on 48 patients diagnosed with IC/BPS-HL and 2 unaffected controls (UC). Thin (4–6 µm) cross sections of bladder tissue were then stained with either H&E or specific CD markers. Images of Hunner lesions and control tissue are shown. B Average cell marker counts found in the bladder biopsy samples of patients with IC/BPS-HL vs. UC. Cells positive for each marker were quantified per high-dry field (×400), taken in the area of maximum diffuse infiltration by the inflammatory cells. Data are expressed as mean ± SEM. C Relative proportions of cells displaying each cell marker in UC and IC/BPS-HL cohorts. The average proportion of Cluster of Differentiation (CD)-20 positive (CD20+) B cells (IC/BPS-HL: 12%; UC: 2%) and CD138+ plasma cells (IC/BPS-HL: 21%; UC: 2%) was much greater in pooled samples from patients with IC/BPS-HL compared to pooled samples from unaffected controls, while the percentage of Natural Killer cells (IC/BPS-HL: 7%; UC: 15%), Monocytes (IC/BPS-HL: 18%; UC: 15%), and Neutrophils (IC/BPS-HL: 14%; UC: 13%) did not differ significantly between the two cohorts. The relative abundance of cells displaying the CD3 T cell marker fell from 52% among the unaffected controls to 29% among the IC/BPS-HL cohort

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