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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: GDF11 alleviates neointimal hyperplasia in a rat model of artery injury by regulating endothelial NLRP3 inflammasome activation and rapid re-endothelialization

Fig. 1

GDF11 inhibited NLRP3 inflammasome activation and pyroptosis in the lysoPC-treated human endothelial cells. A Cell viability was detected by CCK-8 assays after treatment with different concentrations of lysoPC for 24 h. B Cytotoxicity was evaluated by LDH release. C, D The effect of lysoPC on the expression of GDF11 in human endothelial cells was detected by Western blotting. E Cells were pretreated with 50 ng/mL GDF11 for 1 h, followed by lysoPC treatment for 24 h. Cell viability was detected by CCK-8 assays. F Cytotoxicity was evaluated by the LDH release. G, H The effect of GDF11 on the expression of the NLRP3 inflammasome (NLRP3, Casp1 p20, and IL-1β) and pyroptosis executive protein (GSDMD-N) in human endothelial cells treated with lysoPC was analyzed by Western blotting. I, J Photomicrographs of double-fluorescent staining with propidium iodide (PI) (red) and Hoechst 33,342 (blue) (scale bar represents 100 µm). * p < 0.05 vs. the control group; # p < 0.05 vs. the lysoPC group

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