Fig. 7

MiR-30c-5p-EVs suppress the progression of PTC in vitro and in vivo. A Proliferation of PTC cells in each group was detected using colony formation assay (left panel), and the colony number was counted (right panel; n = 3). B The migration properties in each group were determined by using Transwell assay (left panel). Magnification, × 200. Collective analysis of the migration cell number from EVs-treated-PTC cells (right panel; n = 3). C Representative images of xenografts in W3 tumour-bearing nude mice at 28 days postimplantation. D, E Changes in tumor volumes (D) and weight (E) in each W3 xenograft model group. F Changes of miR-30c-5p expression were detected by real-time PCR. G The expression of PELI1 in mice tumor treated with miR-30c-5p-EVs was detected by real-time PCR. H Western blot analysis showing the levels of PELI1 and p-AKT in the tumor samples. I Ki-67 and MMP2 immunohistochemical staining on tumor sections from the NC-EV and miR-30c-5p-EV group (up panel), and the quantification of Ki-67 and MMP2 expression (down panel). The scale bars: 50 µm. *P < 0.05; **P < 0.01, ***P < 0.001