Fig. 4

PELI1 is a direct target of miR-30c-5p in PTC cells. A Overlap of miRNAs targeting PELI1 in the PicTar, miRanda and Targetscan, and miRNAs significantly downregulated in THCA form starBase datasets. B Correlation analysis of miR-30c-5p, miR-30a-5p, miR-301a-3p, miR-454-3p, miR-194-5p, miR-374b-5p, miR-153-3p, and PELI1 in THCA using starBase. C MiR-30c-5p was detected in 12 pairs of PTC tissues by real-time PCR. D Relationship between miR-30c-5p and PELI1 protein in 12 pairs of PTC tissues was measured. E Predicted miR-30c-5p target sequences (red) in PELI1 3′-UTR. Seven nucleotides (*) were mutated to prevent binding to miR-30c-5p. F Relative luciferase activity of reporter plasmids carrying wild-type or mutant PELI1 3′-UTR in PTC cells co-transfected with miR-NC or miR-30c-5p mimics (n = 4). 44% and 41% are the mean reduction values of four duplicate from one experiment and the corresponding P-value is 0.0147 and 0.004 respectively. G Real-time PCR analysis of the expression of PELI1 in PTC cells at 24 h post transfection of the miR-30c-5p mimics or miR-30c-5p inhibitor compared to their negative controls (n = 3). H Level of PELI1 in PTC cells after miR-30c-5p mimics or miR-30c-5p inhibitor transfected was analyzed by western blotting. *P < 0.05, **P < 0.01, ***P < 0.001