Fig. 2

PELI1 overexpression enhances the cellular proliferation, migration and oncogenic transformation in PTC cells. A Western blottings showing the PELI1 levels in lentiviral infected W3 and PTC-1 cells. The results are representative of three independent experiments. B, C CCK8 assay was performed to estimate the cell proliferation of W3 and TPC-1 cells with PELI1 overexpression (B) or knock-down (C) at 24, 48, 72 and 96 h (n = 5). D, E W3 and TPC-1 cells with PELI1 overexpression or PELI1 depletion were subjected to colony-forming assay, the representative micrographs of crystal violet-stained cell colonies were displayed (D), and the quantification of colony formation assays was shown (n = 3) (E). F, G Transwell assay was performed to estimate the cell migration of W3 and TPC-1 cells with PELI1 overexpression or knock-down, the representative images (× 200) of Transwell assays were displayed (F), and the quantification of Transwell assays was shown (n = 3) (G). H, I Representative images (× 100) of scratch wound healing assays of W3 and TPC-1 cells stably expressing PELI1 or knock-down (n = 3). J–L Xenograft model in nude mice and the tumors were showed, and tumor volume (K) and tumor weight (L) were analysis. *P < 0.05, **P < 0.01, ***P < 0.001