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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: GM-CSF impairs erythropoiesis by disrupting erythroblastic island formation via macrophages

Fig. 5

GM-CSF treatment leads to decreased mouse BM EBI formation via decreased CD163 and Vcam1 expression. A Plot of F4/80 versus FSC-H of DAPI−CD11b−Gr1− cells in control and GM-CSF-treated mice BM. B Quantitative analysis of F4/80+ percentage and the fluorescence intensity of F4/80. C P-stat5 expression examined by flowcytometry after GM-CSF stimulation. D Plot of CD163 versus FSC-H of DAPI−CD11b−Gr1− F4/80+macrophages in control and GM-CSF treated mice BM. E Quantitative analysis of CD163+ percentage and the fluorescence intensity of CD163. F Plot of Vcam1 versus FSC-H of DAPI−CD11b−Gr1− F4/80+macrophages in control and GM-CSF-treated mice BM. G Quantitative analysis of Vcam1+ percentage and the fluorescence intensity of Vcam1. H Plot of CD169 versus FSC-H of DAPI−CD11b−Gr1− F4/80+macrophages in control and GM-CSF-treated mice BM. I Quantitative analysis of CD169+ percentage and the fluorescence intensity of CD169. *P < 0.05, ** P < 0.01 *** P < 0.001. N = 3–6

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