Fig. 6

Neat1-mediated binding of EZH2 to the Smad7 promoter region. A, B Expression of Smad7 and p-Smad2/3 was assessed by western blotting and quantified relative to GAPDH and total Smad2/3 expression (n = 5 in each group). C, D Enrichment of EZH2 and H3K27me3 on the Smad7 promoter region determined by ChIP-qPCR (n = 5 in each group). E, F Western blotting analysis and quantification of the expression of Smad7 and p-Sma2/3 (n = 5 in each group). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, NS = no significant difference between the indicated groups