Fig. 7

Bilirubin modifies the composition of T cells in the peripheral blood. After determining the expression of 26 surface markers on the PWB cells using mass cytometry, the T cells expressing CD3 were gated and analyzed using FlowSOM. 29 T cell clusters were identified. A viSNE was used to visualize the distribution of the peripheral blood T cells. 29 T cell clusters were identified and colored on the viSNE map. B A heatmap showing the normalized expression of 12 indicated markers in 29 T cell clusters. C Bar plots showing the frequencies of T7, T9, T13, T17, or T20 in peripheral blood T cells obtained from ApoE^−/− mice treated with or without bilirubin (left panel). D A heatmap showing the Pearson correlation coefficients for relationships between the concentrations of TBIL, LDL, HDL, TG, or TCHO in peripheral blood and the frequencies of peripheral blood T cell clusters. E Dot plots (n = 22) showing the Pearson correlation coefficients for relationships between the concentrations of total bilirubin in peripheral blood and the frequencies of T9 or T20 in peripheral blood T cells. F Dot plots showing the Pearson correlation coefficients for relationships between the concentrations of LDL in peripheral blood and the frequencies of T7, T9, T13, or T17 in peripheral blood T cells. Error bars represent mean ± SD. Dots represent individual samples, n (control) = 8, n (bilirubin) = 14. *P < 0.05, **P < 0.01. Differences were determined by an unpaired t-test. Correlations were determined by a Pearson test