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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: CTPS1 promotes malignant progression of triple-negative breast cancer with transcriptional activation by YBX1

Fig. 4

YBX1 directly binds to the CTPS1 promoter to regulate CTPS1 expression in TNBC cells. a Dual-luciferase reporter assay confirmed the interaction of YBX1 and CTPS1 promoter in MDA-MB-231 cells. b CTPS1 mRNA expression level was identified by qRT-PCR after overexpression of YBX1. c The binding site of YBX1 and CTPS1 was predicted by JASPAR database. d Diagrammatic drawing of CTPS1 promoter fragments. e Luciferase activity of different CTPS1 promoter fragment was detected after YBX1 overexpression. f, g Luciferase activity of CTPS1 promoter (WT or MUT) was detected after YBX1 overexpression in MDA-MB-231 cells. h ChIP assay verified that YBX1 could bind to the CTPS1 promoter in MDA-MB-231 cells. All data are presented as the mean ± SD of three independent experiments. ** p < 0.01

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