Fig. 4

NQO1 overexpression upregulated renal Sirt1 expression and activity in db/db mice. A, B The expression of Sirt1 was measured by western blotting (n = 6) and RT-qPCR (n = 6). (C) Sirt1 activity in renal tissues (n = 6). D Sirt1 was measured by immunohistochemical staining in db/db mice (scale bar, 50 μm, n = 9). E The intracellular NAD+/NADH ratio was measured in db/db mice (n = 6). Sirt1: Silencing information regulator 1. Values are expressed as the mean ± SD. **P < 0.01 versus the db/m group; ^##P < 0.01 versus the db/db + AAV-Control group