Fig. 2

Analysis of differentially expressed exosomal circRNAs. Microarray analysis was performed to screen the differentially expressed exosomal circRNAs in three GDM patients during the third trimester 48 h before delivery, compared with that 48 h after delivery. A heat map for the differentially expressed exosome-derived circRNAs in GDM plasma before delivery by hierarchical clustering. The expression profiles of differentially expressed circRNAs could distinguish the GDM exosome samples before delivery and after delivery. B the differentially expressed circRNAs were analyzed by Venn diagram analysis. Total 152 overlapped circRNAs were differentially expressed in three paired samples. C circRNAs of interest were verified by RT-qPCR analysis in three paired GDM samples. D–J the repeatability of seven interesting circRNAs were verified by RT-qPCR analysis for the third-trimester GDM cohort. *P < 0.05,**P < 0.01,***P < 0.001, ns, no significant difference