Fig. 2

Evaluation of the long-term stability of the cryopreserved CAR/TCR T-cell flow cytometry quality controls (closed-vial stability). The cryopreserved flow cytometry quality control cells were thawed at 2 weeks, 1 month, 2 months, 3 months, 6 months, 9 months and 12 months and stained. The viability, CD3, CD4 and CD8 frequencies as well as surface CAR/TCR T-cell markers: protein L, CD22-Fc (Siglec-2) and CD19-Fc for CD19/CD22 bispecific CAR T-cell (A), EGFR for FGFR4 CAR-T cell (B) and murine TCR-beta for KK-LC-1 TCR T-cell (C) were measured by flow cytometry. Data are shown as mean ± SD. The upper and lower bounds (± 20% range of values measured at time of cryopreservation) are also shown in each plot