Fig. 3

Evolution of TRPC1 and TRPC3 mRNA and protein expression levels in EDL muscles from WT and DMD^mdx rats during post-natal development. A and B Relative levels of TRPC1 and TRPC3 mRNAs expression in EDL muscle extracts from WT and DMD^mdx rats of 1.5 to 7 months of age. Results were normalized to HPRT1 mRNA expression. C and D Relative levels of protein expression of TRPC1 and TRPC3 in EDL muscle homogenates from WT and DMD^mdx rats of 1.5–7 months of age. Representative films exposed to immunoblots are reported below histograms (obtained for muscle extracts from 6 WT and 6 DMD^mdx rats of 1.5 months of age). A–D Each bar represents mean value ± SEM calculated for N animals (N indicated at the bottom of the corresponding bar). For each age and genotype, immunoblots were realized in a minimum of 3 replicates. *: significantly different from mean value measured in age-matched WT animals. $: significantly different from mean value measured in animals from the same genotype of 1.5 months of age. P < 0.05, Kruskal–Wallis and bilateral Conover-Iman post-hoc test