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Table 6 Accuracy test as measured by spike recovery of reduced hIL-33 in matrices using reduced hIL-33 iPCR assay

From: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

hIL-33 (pg/mL) VH
(1:2)
AH
(1:2)
Serum
(1:4)
Plasma
(1:2)
NF
(1:2)
Endogenous conc < 0.078 0.59 2.24 5.73 7.91
Spiked conc. (low) 0.47 0.47 0.45 0.36 0.45
Measured conc 0.41 1.14 3.14 7.26 9.78
Expected conc. (endo + spike) 0.47 1.06 2.69 6.09 8.36
% Recovery 86.5 107.1 117.0 119.2 117.0
Endogenous conc < 0.078 0.59 2.24 4.78 7.91
Spiked conc. (mid) 1.11 1.36 1.51 1.40 1.51
Measured conc 0.93 1.73 3.48 5.90 11.0
Expected conc. (endo + spike) 1.11 1.95 3.75 6.18 9.42
% Recovery 84.1 88.7 92.8 95.5 116.8
Endogenous conc < 0.078 3.21 2.24 5.73 7.91
Spiked conc. (high) 4.25 4.25 4.48 5.22 4.48
Measured conc 3.49 6.9 5.44 10.48 14.7
Expected conc. (endo + spike) 4.25 7.47 6.72 10.95 12.39
% Recovery 82.1 92.4 81.0 95.8 118.3