Table 6 Accuracy test as measured by spike recovery of reduced hIL-33 in matrices using reduced hIL-33 iPCR assay
hIL-33 (pg/mL) | VH (1:2) | AH (1:2) | Serum (1:4) | Plasma (1:2) | NF (1:2) |
|---|---|---|---|---|---|
Endogenous conc | < 0.078 | 0.59 | 2.24 | 5.73 | 7.91 |
Spiked conc. (low) | 0.47 | 0.47 | 0.45 | 0.36 | 0.45 |
Measured conc | 0.41 | 1.14 | 3.14 | 7.26 | 9.78 |
Expected conc. (endo + spike) | 0.47 | 1.06 | 2.69 | 6.09 | 8.36 |
% Recovery | 86.5 | 107.1 | 117.0 | 119.2 | 117.0 |
Endogenous conc | < 0.078 | 0.59 | 2.24 | 4.78 | 7.91 |
Spiked conc. (mid) | 1.11 | 1.36 | 1.51 | 1.40 | 1.51 |
Measured conc | 0.93 | 1.73 | 3.48 | 5.90 | 11.0 |
Expected conc. (endo + spike) | 1.11 | 1.95 | 3.75 | 6.18 | 9.42 |
% Recovery | 84.1 | 88.7 | 92.8 | 95.5 | 116.8 |
Endogenous conc | < 0.078 | 3.21 | 2.24 | 5.73 | 7.91 |
Spiked conc. (high) | 4.25 | 4.25 | 4.48 | 5.22 | 4.48 |
Measured conc | 3.49 | 6.9 | 5.44 | 10.48 | 14.7 |
Expected conc. (endo + spike) | 4.25 | 7.47 | 6.72 | 10.95 | 12.39 |
% Recovery | 82.1 | 92.4 | 81.0 | 95.8 | 118.3 |