Table 5 Dilution linearity of reduced hIL-33 endogenously expressed in human matrices
Dilution factor in matrix | Observed (pg/mL) | Corrected (pg/mL) | % Recovery |
|---|---|---|---|
1:2 VH | 3.44 | 6.87 | 110.7 |
1:4 VH | 1.45 | 5.78 | 93.1 |
1:8 VH | 0.75 | 5.98 | 96.2 |
1:2 AH | 3.21 | 6.43 | 98.3 |
1:4 AH | 1.66 | 6.65 | 101.7 |
1:2 serum | 3.02 | 6.04 | 66.3 |
1:4 serum | 2.24 | 8.94 | 98.2 |
1:10 serum | 1.07 | 10.70 | 117.5 |
1:20 serum | 0.54 | 10.75 | 118.0 |
1:2 plasma | 4.77 | 9.55 | 96.4 |
1:4 plasma | 2.79 | 11.14 | 112.5 |
1:10 plasma | 1.03 | 10.27 | 103.7 |
1:20 plasma | 0.43 | 8.64 | 87.3 |
1:2 NF | 7.91 | 15.82 | 109.1 |
1:4 NF | 3.94 | 15.77 | 108.7 |
1:10 NF | 1.32 | 13.16 | 90.7 |
1:20 NF | 0.66 | 13.29 | 91.6 |