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Table 2 Standard curves performance of reduced and total hIL-33 ELISA

From: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

 

Nominal conc (pg/mL)

Measured mean conc (pg/mL)

% Recovery

% CV

Reduced hIL-33 ELISA

1000

991.5

99.2

1.6

400

408.2

102.1

2.8

160

158.5

99.0

1.7

64

63.6

99.4

1.5

25.6

26.2

102.3

7.0

10.2

11.0

107.6

5.6

4.10

3.83

93.6

9.1

Total hIL-33 ELISA

1000

1007.4

100.7

0.7

400

407.5

101.9

2.5

160

159.6

99.7

2.4

64

64.7

101.1

8.4

25.6

27.6

107.9

8.4

10.2

9.56

93.4

9.6

4.10

4.54

110.8

10.5

  1. n = 6 independent assay runs. LLOQ = 4.1 pg/mL, ULOQ = 1000 pg/mL