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Table 2 Standard curves performance of reduced and total hIL-33 ELISA

From: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

  Nominal conc (pg/mL) Measured mean conc (pg/mL) % Recovery % CV
Reduced hIL-33 ELISA 1000 991.5 99.2 1.6
400 408.2 102.1 2.8
160 158.5 99.0 1.7
64 63.6 99.4 1.5
25.6 26.2 102.3 7.0
10.2 11.0 107.6 5.6
4.10 3.83 93.6 9.1
Total hIL-33 ELISA 1000 1007.4 100.7 0.7
400 407.5 101.9 2.5
160 159.6 99.7 2.4
64 64.7 101.1 8.4
25.6 27.6 107.9 8.4
10.2 9.56 93.4 9.6
4.10 4.54 110.8 10.5
  1. n = 6 independent assay runs. LLOQ = 4.1 pg/mL, ULOQ = 1000 pg/mL