Fig. 5
Escherichia coli contributes to osteolytic bone lesion in a mouse model of myeloma in complete remission. A C57BL/KaLwRij mice were intravenous injected with the 5TGM1 (5 × 105 cells/mouse) with or without E. coli (1 × 104 cells/mouse). After 4 weeks, 1 mg/kg bortezomib and 2 mg/kg melphalan were injected intraperitoneally into the mice (3 times a week) for 2 weeks. Histomorphometric analysis of BV/TV (B), OS/BS (C), Ob.S/BS (D), ES/BS (E) and Oc.S/BS (F). G, H Bone formation rate (BFR/BS) was measured by calcein injection, and the bone sections were imaged and analyzed. Shown are representative images and summarized data of bone formation in mouse femurs. Scale bar: 20 μm. Data are means ± SD (n = 5 mice/group, three replicate studies). *P < 0.05; **P < 0.01. P values were determined using one-way ANOVA