Fig. 2

LOX expressed by CAFs affects the biological characteristics of OSCC cells. A Gene expression of LOX in three paired NFs and CAFs measured by RT-qPCR. B Protein levels of LOX in three paired NFs and CAFs determined by western blot. C CCK-8 assay was used to evaluate the proliferation ability of Cal27 and HN6 cells. D The invasion ability in Cal27 and HN6 cells were evaluated by transwell assay. Representative images of invaded cells and quantification data were shown. E The migration ability in Cal27 and HN6 cells were detected by the scratch test. Representative images were shown. F Expression of EMT markers in Cal27 and HN6 cells were examined by western blot. G Quantitation of EMT markers mRNA levels were determined by RT-qPCR. GAPDH served as loading control; β-actin served as loading control. The data are presented as the means ± SD (n = 3); *P < 0.05, **P < 0.01, ***P < 0.001