Fig. 5
From: SIRT5 functions as a tumor suppressor in renal cell carcinoma by reversing the Warburg effect
SIRT5 decelerates cell proliferation and inhibits cell migration through PDHA1. A Growth curves of Caki-1 cells overexpressing or silenced for SIRT5 were determined. B PDHA1 was knocked down in Caki-1 cells, and then the wild-type and K351Q mutant (PDHA1K351Q) PDHA1 plasmids were reintroduced into the cells. The growth curves of two modified Caki-1 cell clones with or without SIRT5 expression were compared. Growth curves (C) and (D) clone formation assays 786-O cells with SIRT5 knockdown were determined. E Transwell invasion assay using wild-type and shSIRT5-expressing Caki-1 and 786-O cells. F Wild-type and mutant PDHA1K351Q PDHA1 were introduced into Caki-1 cells, and photos of cells with or without SIRT5 expression were captured at 0, 6, and 24 h. G Wound healing assay of SIRT5 knockdown in 786-O cells. H Representative images of two different ccRCC organoids transfected with the shCtrl or shSIRT5 lentivirus and quantification of organoid diameters