Fig. 4

SIRT5 regulates PDHA1 activity and remodels metabolic pathways. A Succinyl-CoA was added to the PDHA1 system, and the levels of succinylated PDHA1 and PDHA1 enzyme activity were determined. The levels of succinylated PDHA1 and PDHA1 enzyme activity were determined following the overexpression (B) or knock down of PDHA1 (C). D OCRs were detected for untransfected and SIRT5-transfected KMRC-20 cells that were treated with DMHG. OCRs were recorded from cells treated with oligomycin, carbonyl cyanide-m-chlorophenylhydrazone (FCCP), and antimycin A/rotenone at the indicated time points. E SIRT5 was knocked down in Caki-1 cells, and the OCR was detected and quantified. F The levels of key metabolites produced by glycolysis and the TCA cycle were determined following SIRT5 overexpression or knockdown