Fig. 3

TRIM38 inhibition enhanced BLCA proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 in T24 and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm