Fig. 5

Knockdown of IRAK1 induces apoptosis of and radiosensitized EC KYSE30 cells. The radioresistant KYSE30 (KYSE30R) cell line was treated with sh-IRAK1. A IRAK1 knockdown in radioresistant KYSE30 cells was determined using Western blot analysis, normalized to GAPDH. B Colony-formation ability of irradiated (8 Gy)/unirradiated (0 Gy) radioresistant KYSE30 cells with IRAK1 knockdown under 0/8 Gy X-ray irradiation was detected by colony formation assay. C Apoptosis of radioresistant KYSE30 cells with IRAK1 knockdown under 8 Gy X-ray or under no irradiation detected by flow cytometry based Annexin V-FITC/PI apoptosis assay. D Expression of cleaved-caspase3, cleaved-caspase8, pro caspase3 and pro caspase8 in radioresistant KYSE30 cells with IRAK1 knockdown under 8 Gy X-ray or under no irradiation measured using Western blot analysis, normalized to GAPDH. Values obtained from three independent experiments in triplicate were expressed as mean ± standard deviation. Values between two groups were analyzed by unpaired t-test. * p < 0.05