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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Promotion of microRNA-146a by histone deacetylase 4 silencing contributes to radiosensitization of esophageal carcinoma

Fig. 4

IRAK1 is highly expressed in radioresistant EC tissues and cells and is targeted by miR-146a. A Target genes of miR-146a predicted using microDB, mirDIP, and other databases. The five ellipses in the graph represent the prediction results of five databases, and the middle part represented the Venn intersection of the prediction results in five databases. B Analysis of IRAK1 expression in ESCC in TCGA database. The abscissa refers to the type of sample, and the ordinate is the level of gene expression. The red box represents the tumor sample, and the gray box represents the normal sample. * p < 0.01. C IRAK1 expression in radioresistant (n = 46) and radiosensitive ESCC (n = 36) tissues was determined using Western blot analysis, normalized to GAPDH. D IRAK1 expression in KYSE30 and radioresistant KYSE30 (KYSE30R) cells was determined using Western blot analysis, normalized to GAPDH. E Putative miR-146a binding sites in the IRAK1 mRNA 3’UTR. F IRAK1 as the target of miR-146a was evaluated using dual-luciferase reporter gene assay. G IRAK1 expression in radioresistant KYSE30 cells with elevated miR-146a was determined using Western blot analysis, normalized to GAPDH. Values obtained from three independent experiments in triplicate were expressed as mean ± standard deviation. Values between two groups were analyzed by unpaired t-test. * p < 0.05 compared with radiosensitive ESCC tissues, KYSE30 cells, or radioresistant KYSE30 cells treated with mimic NC. #p < 0.05 compared with radioresistant KYSE30 cells treated with inhibitor NC

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