Fig. 2

Silencing of HDAC4 induces apoptosis and radiosensitivity of EC cells. Radioresistant KYSE30 (KYSE30R) cells were transduced with HDAC4 overexpression (oe-) plasmids or plasmids containing shRNA (sh-) targeting HDAC4 under X-ray (8 Gy) or under no irradiation (0 Gy). A HDAC4 overexpression and knockdown in radioresistant KYSE30 cells was verified using Western blot analysis, normalized to GAPDH. B Colony-formation ability of radioresistant KYSE30 cells was detected by colony formation assay in response to HDAC4 overexpression and knockdown. C Apoptosis of radioresistant KYSE30 cells was detected by flow cytometry based Annexin V-FITC/PI apoptosis assay in response to HDAC4 overexpression and knockdown. D Expression of cleaved-caspase3, cleaved-caspase8, pro caspase3 and pro caspase8 in radioresistant KYSE30 cells with HDAC4 overexpression and knockdown was measured using Western blot analysis, normalized to GAPDH. Values obtained from three independent experiments in triplicate were expressed as mean ± standard deviation and values between two groups were analyzed by unpaired t-test. * p < 0.05