Fig. 2

Ligand-receptor pair interaction strength calculation and analysis. A t-SNE plot showing clusters of 6 main cell types from 25 CRC scRNA-seq samples. B Description of the strength of 1733 ligand-receptor pairs’ interactions strength. The heatmap shows ligand-receptor pairs’ interaction strength between 6 main classes and 25 subclasses cells. The density plot shows average interaction strength between the different cell types. C Network plot diagram shows ligand-receptor pair interactions in 24 cell types. D CSOmap was used to reconstruct the three-dimensional spatial structure of the 25 CRC scRNA-seq samples. E Diagrams and density plot of different longitudinal sections of tumor tissue spatial structure. F The statistical significance of interactions between different cell types. G Proportion of cell types away from the interaction center. H Functional enrichment of the downregulated DEGs in malignant epithelial cells far from the interaction center