Fig. 1

The effect of sPLA2-IIA on cholesterol efflux capacity and relative gene expression. A Cholesterol efflux capacity was significantly increased by sPLA2-IIA and GW9662 reversed the cholesterol efflux capacity. B ABCA1 mRNA expression was up-regulated in a dose-dependent manner. *p = 0.025, **p = 0.0424, NS = no significance, &p = 0.0002 versus control, #p = 0.0073 versus control, n = 3. Transcriptions levels of C ABCA1, D LXR-α, and E PPAR-γ in control, sPLA2-IIA and sPLA2-IIA (300 ng/ml) + GW9662 (16 μM) groups. #p = 0.0383, $p = 0.0081, **p = 0.006, &p = 0.0075, *p = 0.0195, ***p = 0.001, n = 3. Western blot results of PPAR-γ, LXR-α and ABCA1 protein expression in the control, sPLA2-IIA, sPLA2-IIA + GW9662 macrophages after co-incubation for 48 h. F A represented the blots of PPAR-γ, LXR-α and ABCA1 lanes. There were significantly greater amounts of G PPAR-γ, H LXR-α, and I ABCA1 in sPLA2-IIA macrophages compared with those in the control. Under the extra treatment of GW9662, the increased amounts of PPAR-γ, LXR-α, and ABCA1 were markedly reversed. *p = 0.000, **p = 0.0024, n = 3