Fig. 4

The proinflammatory ATM phenotype was reduced in HFD-fed CD226KO mice. A Serum concentrations of M1-type cytokines and chemokines in WT and CD226KO mice fed with chow or a HFD for 16 weeks (n = 6). B FACS analysis of M1-type (CD11c⁺) ATMs from WT and CD226KO mice with a HFD (n = 4). C mRNA levels of M1/M2-type markers and chemokines in epididymal ATMs from HFD WT and CD226KO mice (n = 3). D Glycolytic activity of ATMs from HFD WT and CD226KO mice (n = 3) Combined data from three independent experiments. 2-DG, 2-deoxyglucose. Data represent mean ± SEM. Differences between groups were determined by one way ANOVA with Tukey’s multiple test (A, C, D) or unpaired Student’s t-test (B). *P < 0.05, **P < 0.01