Fig. 3

Decrease of ATMs in HFD-fed CD226KO mice. A FACS analysis of F4/80⁺ macrophages in the spleens of WT and CD226KO mice fed with either chow or a HFD for 16 weeks (n = 3). B FACS analysis of F4/80⁺ macrophages in epididymal SVF (n = 4). C Representative images and quantification of immunofluorescence staining of F4/80 in the epididymal fat of WT and CD226KO mice (n = 4). Scale bar means 100 μm. D Gross images and absolute cell count of epididymal SVF cells and F4/80⁺ macrophages obtained from groups of mice fed with chow or a HFD (n = 4). Combined data from three independent experiments. Data represent mean ± SEM. Differences between groups were determined by one way ANOVA with Tukey’s multiple test (A, B) or unpaired Student’s t-test (D). *P < 0.05, **P < 0.01