Fig. 5
From: LINC00491 promotes cell growth and metastasis through miR-324-5p/ROCK1 in liver cancer
LINC00491 promoted ROCK1 expression by reducing miR-324-5p. A The targeting regions of miR-324-5p for ROCK1. B Dual luciferase reporter gene assay was performed using HEK-293T cells. Relative luciferase activity in HEK-293T cells co-transfected with ROCK1-WT or ROCK1-MUT luciferase reporter and miR-324-5p NC or mimics. C The expression of ROCK1 in 60 cases of liver cancer and adjacent tissue samples. D, E The effect of miR-324-5p on the mRNA (D) and protein (E) expression of ROCK1 in HUH-7 and SK-Hep-1 cell. F Dual luciferase reporter gene assay was performed using HEK-293T cells. Relative luciferase activity in HEK-293T cells co-transfected with ROCK1-WT or ROCK1-MUT luciferase reporter and LINC00491 over-expression vector (LINC00491) or siLINC00491. G, H The effect of LINC00491 over-expression vector (LINC00491) and siLINC00491 on the mRNA (G) and protein (H) expression of ROCK1 in HUH-7 and SK-Hep-1 cell. ns: the difference was not statistically significant