Fig. 5

HCG18 regulates CD28 expression by sponging miR-145-5p in a ceRNA manner. a Expression levels of CD28 mRNA were examined by RT-PCR after transfection with negative control or miR-145-5p mimics in Jurkat cells. b Expression levels of CD28 protein were examined by western blotting after transfection with negative control or miR-145-5p mimics in Jurkat cells. c Expression levels of CD28 mRNA were examined by RT-PCR analysis after transfection with negative control, shHCG18, and shHCG18 + miR-145-5p inhibitor in Jurkat cells. d Expression levels of CD28 protein were examined by western blotting after transfection with negative control, shHCG18 and shHCG18 + miR-145-5p inhibitor in Jurkat cells. The experiment was repeated at least three times, and data are presented as the mean ± SD, ****p < 0.0001