Fig. 4

Upregulation of HCG18 is a target of miR-145-5p in MG. a The expression of HCG18 was examined in MG patients and control subjects by RT-PCR. b Transfection efficiency of miR-145-5p mimics was determined by RT-PCR. c The relative expression level of HCG18 after transfecting with miRNA NC or let-7c-5p mimics into Jurkat cells was measured using RT-PCR. d The putative miR-145-5p binding sequence of the wild type (WT) and mutation (MUT) sequence of HCG18. e The luciferase reporter plasmid containing HCG18-WT or HCG18-MUT was co-transfected with miR-145-5p mimics or miRNA NC into 293 T cells. The ratio of firefly/renilla activities represented luciferase activitie. The experiment was repeated at least three times, and data are presented as the mean ± SD, *p < 0.05, **p < 0.01, ****p < 0.0001