Fig. 6

MiR-365a-3p targets STAT3 in breast cancer cells. A The predicted binding sites of STAT3 and miR-365a-3p. B–D The MDA-MB-231 and MDA-MB-468 cells were treated with miR-365a-3p mimic. B The levels of miR-365a-3p were validated by qPCR. C and D The luciferase activities of STAT3 were determined by luciferase reporter gene assays. E The MDA-MB-231 and MDA-MB-468 cells were treated with circNOLC1 overexpression plasmid and miR-365a-3p mimic. The protein levels of STAT3 were determined by Western blot analysis. F The levels of STAT3 were examined by qPCR in clinical breast cancer tissues (n = 30). **P < 0.01