Fig. 7
From: Circular RNA circPIP5K1A contributes to cancer stemness of osteosarcoma by miR-515-5p/YAP axis
CircPIP5K1A contributes to cancer cell stemness by targeting miR-515-5p/YAP axis in osteosarcoma cells. A–D MG63 and 143B cells were transfected with circPIP5K1A siRNA or miR-515-5p mimic, or co-transfected with circPIP5K1A siRNA and YAP overexpression vector or miR-515-5p inhibitor. A, B The stemness was analyzed by sphere formation assays. C, D The protein levels of ALDH1 and Nanog were detected by Western blot. E The expression of Nanog was measured by immunofluorescence analysis. Mean ± SD (**P < 0.01)