Fig. 6
From: Circular RNA circPIP5K1A contributes to cancer stemness of osteosarcoma by miR-515-5p/YAP axis
YAP is a target of miR-515-5p in osteosarcoma cells. A The interacted binding site between YAP and miR-515-5p. B, C MG63 and 143B cells were transfected with miR-515-5p mimic. B Luciferase activities of YAP or YAP mutant (miR-515-5p binding site) were analyzed by luciferase reporter gene assay. C YAP mRNA expression was detected by qPCR. D YAP protein levels were tested by Western blot analysis in MG63 and 143B cells transfected with circPIP5K1A siRNA, or co-transfected with circPIP5K1A siRNA and miR-515-5p inhibitor. Mean ± SD (**P < 0.01)