Fig. 5From: Circular RNA circPIP5K1A contributes to cancer stemness of osteosarcoma by miR-515-5p/YAP axisMiR-515-5p inhibits stemness of osteosarcoma cells. A–E MG63 and 143B cells were transfected with miR-515-5p mimic. A, B The stemness was analyzed by sphere formation assays. C, D The mRNA expression of ALDH1 and Nanog was determined by qPCR. E, F The protein levels of ALDH1 and Nanog were detected by Western blot. Mean ± SD (**P < 0.01, ***P < 0.001)Back to article page