Fig. 3
From: Circular RNA circPIP5K1A contributes to cancer stemness of osteosarcoma by miR-515-5p/YAP axis
The suppression of circPIP5K1A attenuates stemness of osteosarcoma cells. A–G MG63 and 143B cells were transfected with circPIP5K1A siRNA. A, B The stemness was analyzed by sphere formation assays. C, D The CD133+CD44+ cell population was measured by flow cytometer. E, F The mRNA expression of ALDH1 and Nanog was determined by qPCR. G The protein levels of ALDH1 and Nanog were detected by Western blot. Mean ± SD (**P < 0.01, ***P < 0.001)