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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Bioinformatics analysis reveals a stem cell-expressed circ-Serpine2-mediated miRNA-mRNA regulatory subnetwork in the malignant progression of glioma

Fig. 6

Circ-Serpine2 upregulates KIF20A expression, accelerates the proliferation, invasion, and migration of glioma cells, and inhibits apoptosis by sponging miR-124-3p. A Relative circ-Serpine2, miR-124-3p and KIF20A expression was detected by qRT-PCR. B The circRNA expression levels were assessed by qRT-PCR. GAPDH was used as a cytoplasmic marker, and U6 was used as a nuclear marker. C miR-124-3p and KIF20A expression was detected following the knockdown of circ-Serpine2. D KIF20A expression was detected by Western blot assays after transfection. E Colony formation assays were carried out with cotransfection of circ-Serpine2/miR-124-3p inhibitor or KIF20A plasmid in A172 cells. F A scratch test was used to measure the migration ability of A172 cells after transfection (0 h and 24 h). Pictures were captured under a light microscope at a magnification of 10 × 10. G Cell invasion assays were performed by cotransfecting A172 cells with circ-Serpine2/miR-124-3p inhibitor or KIF20A plasmid. The protein expression of invasion-related proteins was determined by Western blotting. H Apoptosis assays were performed by cotransfecting A172 cells with circ-Serpine2/miR-124-3p inhibitor or KIF20A plasmid. Protein expression of apoptosis-related proteins was determined through Western blotting. *P < 0.05 **P < 0.01

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