Fig. 5

Circ-ERBB2 influenced the proliferation, invasion, migration and apoptosis of SKBR3 and BT-474 cells through the circ-ERBB2/miR-136-5p/TFAP2C axis or the circ-ERBB2/miR-198/TFAP2C axis. si-circ-ERBB2 and/or miR-136-5p inhibitor, si-circ-ERBB2 and/or miR-198 inhibitor were separately transfected into SKBR3 and BT-474 cells. A, B The proliferation of SKBR3 and BT-474 cells was assessed by CCK-8 assay, two-way ANOVA combined with Tukey post-hoc. C–F The migration and invasion of SKBR3 and BT-474 cells were analyzed by Transwell assay (scale bar = 200 μm), D One-way ANOVA combined with LSD post-hoc, F One-way ANOVA combined with LSD post-hoc. G, H The apoptosis of SKBR3 and BT-474 cells was assessed by flow cytometry analysis, H One-way ANOVA combined with LSD post-hoc. I The protein levels of TFAP2C, HER2, Akt, p-Akt, ERK and p-ERK were detected by Western blot. Data are represented as mean ± SD. *P < 0.05, **P < 0.01 or ***P < 0.001 vs. si-NC + inhibitor NC. ^#P < 0.05, ^##P < 0.01 or ^###P < 0.001 vs. si-ERBB2 + inhibitor NC