Fig. 3

Interaction between circ-ERBB2 and miR-136-5p, circ-ERBB2 and miR-198 in SKBR3 and BT-474 cells. A Fluorescence in situ hybridization (FISH) was applied to analyze the localization of circ-ERBB2 in SKBR3 and BT-474 cells (scale bar = 10 μm). B The binding of circ-ERBB2 to Ago2 was verified by RNA immunoprecipitation (RIP), unpaired two-tailed t-test. C, D RNA pull-down assay was conducted to investigate the interaction between circ-ERBB2 and miR-136-5p, miR-564, miR-198, miR-892b, miR-890 and miR-377-3p (all of them are reported in breast cancer and might be regulated by circ-ERBB2), unpaired two-tailed t-test. E Online bioinformatics software (Circular RNA interactome) analysis revealed that there were binding sites between circ-ERBB2 and miR-136-5p. F The interaction between circ-ERBB2 and miR-136-5p was confirmed by dual-luciferase reporter gene assay, unpaired two-tailed t-test. G Online bioinformatics software (Circular RNA interactome) analysis predicted that there were binding sites between circ-ERBB2 and miR-198. H Dual-luciferase reporter gene assay was applied to verify the interaction between circ-ERBB2 and miR-198, unpaired two-tailed t-test. **P < 0.01 vs. mimic NC. Data are represented as mean ± SD. ***P < 0.001 vs. IgG, NC probe or mimic NC. WT: wild type; MUT: mutant type