Fig. 2

Effect of circ-ERBB2 on the proliferation, migration, invasion and apoptosis of SKBR3 and BT-474 cells. A Detection of circ-ERBB2 levels in human breast epithelial cells MCF10A, HER2-positive breast cancer cells SKBR3 and BT-474 and HER2-negative breast cancer cells MDA-MB-231 and MDA-MB-468, one-way ANOVA with Tukey’s post hoc. B, C si-circ-ERBB2 #1, si-circ-ERBB2 #2 and si-circ-ERBB2 #3 were separately transfected into SKBR3 and BT-474 cells, one-way ANOVA with Tukey’s post hoc. The transfection efficiency of si-circ-ERBB2 was assessed by qRT-PCR. D, E si-circ-ERBB2 #2 was transfected into SKBR3 and BT-474 cells. Cell counting kit 8 (CCK-8) assay was applied to analyze the proliferation of SKBR3 and BT-474 cells, two-way ANOVA for group comparison, multiple t-test to compare the same time between two groups. F–I The cell migration and invasion were measured by Transwell assay (scale bar = 200 μm), G, I or J unpaired two-tailed t-test. J Flow cytometry was conducted to detect the apoptosis of SKBR3 and BT-474 cells, values are the mean ± SD of three independent experiments. K, L The protein levels of TFAP2C, HER2 and the downstream signaling pathway proteins Akt, p-Akt, ERK and p-ERK were measured by Western blot analysis. Data are represented as mean ± SD. *P < 0.05, **P < 0. 01, ***P < 0.001 vs. si-NC. ***P < 0.001 vs. MCF10A. NC: negative control