Fig. 5
Comparative assessment of necrotizing enterocolitis (NEC)-like injuries and proportion of lamina propria Tregs between mice that received microbiota from NEC patients or control patients and underwent a NEC-induction protocol. a Histopathological evaluation of inflammation present in intestinal tissues from the GN, GC, GNB and GCB groups (100 × magnification). b NEC score of intestinal tissue injuries [0 (normal ileum) to 4 (loss of intestinal villi with necrosis and transmural necrosis)] observed in the GN, GC, GNB and GCB groups. c Representative flow plot of the Treg population in the lamina propria of mice from GN, GC, GNB and GCB groups. d Percentage of Treg cells in Thelper cells in lamina propria of mice from GN, GC, GNB and GCB groups. GN NEC patient fecal microbiota transplantation (FMT) plus NEC model, GC control patient FMT plus NEC model, GNB butyrate-treated with NEC patient FMT and NEC model, GCB butyrate-treated with control patient FMT and NEC model. GN, n = 10; GC, n = 10; GNB and GCB, n = 11. *p < 0.05; **p < 0.01