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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Effect of the spatial–temporal specific theca cell Cyp17 overexpression on the reproductive phenotype of the novel TC17 mouse

Fig. 6

Estrous cycle and fertility assessment in TC17 mice. During the Dox treatment (8 weeks), the estrous cycle in CTRL and TC17 mice (N = 10) was assessed at 4 weeks after the start of the treatment. Proestrus was categorized by the presence of nucleated and some cornified epithelial cells, estrus by the presence of cornified cells, and metestrus/diestrus for the presence of some cornified epithelial cells and primarily leukocytes. A Percentage of the relative amount of time spent in each cycle stage, B Representative estrous cyclicity of six mice/group during 15 consecutive days. M/D, metestrus/diestrus phase; P, proestrus; E, estrus. C Schematic graphic of the experimental plan for the A separate cohort of female mice of TC17 and CTRL mice at 7-week-old was used to assess fertility. At 8 weeks of age, 1 week after treatment (N = 10) were paired with adult C57BL/6 N breeder males (3-months-old). Breeder males were removed after 10 days. Females were assessed for pregnancy rate (D), time (days) to first litter (E), and a number of pups/litter, means ± s.e.m. Data were analyzed using the two-tailed Mann–Whitney test (*p < 0.05)

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