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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Effect of the spatial–temporal specific theca cell Cyp17 overexpression on the reproductive phenotype of the novel TC17 mouse

Fig. 2

TC17 validation in vivo. Treatment started at 8 weeks old mice. A After Dox treatment (4 weeks), CTRL and TC17 mice were sacrificed, and ovaries were collected (N = 4). RNAscope was performed for Cyp17 probe and Draq5 to stain DNA. Representative confocal micrographs of CTRL (upper panel) and TC17 transgenic mouse ovaries (lower panel). Panels show the effects of Dox treatment in the Cyp17 expression (in red). An antisense control probe was used as control from the vendor. B qPCR validation of Cyp17 expression in TC17 ovaries compared with CTRL ovaries (N = 6). After Dox treatment (8 weeks), ovaries were collected, and qPCR was performed Graphs show fold change means ± s.e.m relative expression to Cyp17 following normalization to the housekeeping gene. Data were analyzed using the two-tailed Mann–Whitney test (***p < 0.001)

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